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| 鸡α干扰素基因遗传转化烟草研究 | |
| 引用本文： | 宋莉,赵德刚,吴拥军.鸡α干扰素基因遗传转化烟草研究[J].云南植物研究,2009,31(3):247-252. |
| 作者姓名： | 宋莉 赵德刚 吴拥军 |
| 作者单位： | 1. 贵州省农业生物工程重点实验室,贵州,贵阳,550025;教育部绿色农药与农业生物工程重点实验室,贵州,贵阳,550025 2. 贵州省农业生物工程重点实验室,贵州,贵阳,550025;贵州大学生命科学学院,贵州,贵阳,550025 3. 贵州大学生命科学学院,贵州,贵阳,550025 |
| 基金项目： | Foundation items： The National Key Projects in the Science ＆ Technology Pillar Program of China （No. 2007BAD59B06）, and the International Science and Technology Cooperation Program of China （No. 2007DFA31260）.Acknowledgements： We thank The Center of Stem Cell and Tissue Engineering Reaserch, Guiyang Medical College, for techni- cal assistance with the eytopathlc effect inhibition analysis. |
| 摘 要： | 利用植物生物反应器生产外源药用蛋白近年来备受关注，本研究通过农杆菌介导法，将人工合成的鸡α干扰素基因（ChIFN-α）转化烟草（Nicotiana tabctcum）无菌苗叶盘。对抗性植株进行的GUS活性鉴定，PCR和RT-PCR检测表明，ChIFN-α基因已整合到烟草基因组中并具有转录活性，ELISA检测和细胞病变（CPE）抑制试验表明转基因烟草表达的干扰素蛋白具有抗病毒活性。 |
| 关 键 词： | 鸡α干扰素 烟草 表达 生物学活性 |
Expression of Bioactive Chicken Alpha Interferon in Transgenic Tobacco |
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| SONG Li,ZHAO De-Gang,WU Yong-Jun.Expression of Bioactive Chicken Alpha Interferon in Transgenic Tobacco[J].Acta Botanica Yunnanica,2009,31(3):247-252. | |
| Authors: | SONG Li ZHAO De-Gang WU Yong-Jun |
| Affiliation: | SONG Li, ZHAO De-Gang, WU Yong-Jun(1 Guizhou Key Laboratory of Agricultural Bioengineering, Guiyang 550025, China; 2 Key Laboratory of Green Pesticide arul Agricultural Biological Engineering , Ministry of Education, Guiyang 550025, China; 3 College of Life Sciences, Guizhou University, Guiyang550025, China) |
| Abstract: | o. Since interferon was involved in host antiviral response, our results provided the possibility for further study of plant oral adjuvant with ChIFN-α for improving immunity in birds. |
| Keywords: | Tobacco Expression Bioactivity |
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