棉铃虫AChE不同分子型对抑制剂的敏感度及其与抗药性的关系(英文)

在分离到的棉铃虫AChE五种不同的分子型中 ,2 .1s和 8.7sAChE抗性品系对毒扁豆碱的敏感度明显低于敏感品系 ,成虫头部I50 值分别相差 1 86.3和 84.8倍 ,幼虫I50 值分别相差 1 0 1 0 倍和 1 0 5 倍。幼虫 5.3sAChE对毒扁豆碱的敏感度抗性品系和敏感品系相差达 1 2 3倍 ,而成虫则没有差异。研究结果表明 2 .1s、5.3s和 8.7sAChE敏感度降低可能是造成棉铃虫对有机磷和氨基甲酸酯类杀虫药剂产生抗性的主要原因

A COMPARISON OF SENSITIVITY TO INHIBITOR AMONG ACETYLCHOLINESTERASE (AChE) MOLECULAR FORMS OF RESISTANT AND SUSCEPTIBLE STRAINS IN HELICOVERPA ARMIGERA

The sensitivity of 2.8s and 8.7s acetylcholinesterase (AChE) to eserine sulfate is significantly lower in resistant (R) strain than in susceptible (S) strain in five AChE forms isolated by sucrose gradient centrifugation from cotton bollworm, Helicoverpa armigera . There are 186 and 85 times of difference in heads of adults and 10 10 and 10 5 times of difference in heads of larvae based on a comparison of I 50 values for 2.8s and 8.7s forms respectively. The sensitivity of 5.3s form of AChE to eserine sulfate shows 123 times of difference between R and S strains in larvae, however no difference in adults. The above results indicate that insensitive 2.8s, 8.7s and 5.3s forms of AChE may play an important role in the resistance of cotton bollworm to organophosphate and carbamate insecticides.