Fluorescein-labeled stable neurotensin derivatives. |
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Authors: | Veronique Maes Christina Hultsch Suzann Kohl Ralf Bergmann Thomas Hanke Dirk Tourwé |
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Institution: | Organic Chemistry Department, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium. |
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Abstract: | Neurotensin(8-13) analogs containing a glycine or 5-aminovaleroyl spacer were labeled with fluorescein through formation of an N-terminal thiourea function. The receptor binding was measured in HT-29 cell cultures and showed a substantial decrease in affinity, especially for the metabolically stabilized MeArg(9), Tle(11)] analog. Using fluorescence microscopy, the internalization of the fluorescent neurotensin analogs into HT-29 cells was observed. |
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Keywords: | neurotensin fluorescein receptor binding fluorescence microscopy |
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