Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1 |
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| Authors: | Ward Donald E Revet Ingrid M Nandakumar Renu Tuttle Jon H de Vos Willem M van der Oost John DiRuggiero Jocelyne |
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| Affiliation: | Laboratory for Microbiology, Wageningen University, 6703CT Wageningen, The Netherlands. deward@unity.ncsu.edu |
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| Abstract: | We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication. |
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