Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution |
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Authors: | Matthew S Kokotilo Jodi Carter Aducio Thiesen Ming H Chen Angela Ochs Rachel G Khadaroo Thomas A Churchill |
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Institution: | aDepartment of Surgery, University of Alberta, Edmonton, Alberta, Canada T6G 2B7;bDepartment of Laboratory Medicine and Pathology, University of Alberta, Canada |
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Abstract: | IntroductionOur lab has developed an effective nutrient-rich solution that facilitates energy production and control of oxidative stress during static cold storage of the intestine; however, the requirement for oncotic agents, such as hydroxyethylstarch (HES), has not been evaluated. This study investigated the effectiveness and requirement for HES in an intraluminal preservation solution during a clinically relevant period of cold storage.MethodsRat intestines were procured, including an intravascular flush with University of Wisconsin solution followed by a ‘back table’ intraluminal flush with a nutrient-rich preservation solution containing varying amounts of HES (n = 6 per group): Group 1, 0%; Group 2, 2.5%; Group 3, 5%; Group 4, 10%. Energetics, oxidative stress, and morphology were assessed over a 24 h time-course of cold storage.ResultsOverall, the 5% HES solution, Group 3, demonstrated superior energetic status (ATP and total adenylates) compared to all groups, P < 0.05. Malondialdehyde levels indicated a reduction in oxidative stress in Groups 3 and 4 (P < 0.05). After 12 h, median modified Parks’ grades for Groups 2 and 3 were significantly lower than Groups 1 and 4, P < 0.05.ConclusionOur data suggests that when employing an intraluminal preservation solution for static organ storage, oncotic support is a fundamental requirement; 5% HES is optimal. |
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Keywords: | Intraluminal preservation solution Intestinal-specific Oncotic/osmotic agents Organ preservation Energetic stress Oxidative stress Osmotic stress |
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