| NASBA——一种新型禽流感病毒检测方法 |
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| 引用本文： | 刘乐庭,刘爽,封燕芸,陈建国,于常海. NASBA——一种新型禽流感病毒检测方法[J]. 中国生物工程杂志, 2005, 25(11): 96-99 |
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| 作者姓名： | 刘乐庭 刘爽 封燕芸 陈建国 于常海 |
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| 作者单位： | 1. 北京大学神经科学研究所北京大学医学部神经生物学系教育部神经科学重点实验室, 北京 100083;2. 香港基因晶片开发有限公司, 香港;3. 北京大学生命科学院, 北京 100871 |
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| 基金项目： | 国家自然科学基金资助项目(30270426,30470543)，北京市自然科学基金资助项目(7032026,7051004) |
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| 摘 要： | NASBA(nucleic acid sequence-based amplification)是一项持续等温的核酸扩增技术,特别适合于以RNA为模版的扩增,与其它常用禽流感病毒检测方法(病毒培养法、免疫学方法和PCR)相比,具有灵敏度高、特异性强、操作简便等特点。就NASBA的操作原理及其在禽流感病毒检测中的成功应用进行综述。NASBA不仅成为禽流感病毒检测的有力工具,而且对于其它恶性传染病的监测、检测同样具有重要价值和意义。
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| 关 键 词： | NASBA 禽流感 检测方法 |
| 收稿时间： | 2005-07-15 |
| 修稿时间： | 2005-08-09 |
NASBA--A New Method to Detect Avian Influenza Virus |
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| LIU Le-ting,LIU Shuang,FENG Yan-yun,CHEN Jian-guo,YU Chang-hai. NASBA--A New Method to Detect Avian Influenza Virus[J]. China Biotechnology, 2005, 25(11): 96-99 |
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| Authors: | LIU Le-ting LIU Shuang FENG Yan-yun CHEN Jian-guo YU Chang-hai |
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| Affiliation: | 1 .Neurescience Research Institute; Department of Neurobiology, Health Science Center; Key Laboratory of Neuroscience, Ministry of Education Peking University Beijing 100083, China;2 .Hong Kong DNA Chips Ltd Hong Kong,China; 3. College of Life Science, Peking University Beijing 100871, China |
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| Abstract: | NASBA (nucleic acid sequence-based amplification) is a continuous, isothermal, enzyme-based method for the amplification of nucleic acid. It is especially suitable for amplifying RNA sequences, and more sentive, specific and convenient than other virus detection methods such as viral culture, viral antigen detection techniques and PCR . In this review, The principle and successful application of NASBA in avian influenza virus detection were focused on. NASBA is not only a useful tool for avian influenza virus detection, but also suitable for routine screening for other RNA virus. |
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| Keywords: | NASBA Avian influenza Detection method |
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