Use of cyanobacterial gas vesicles as oxygen carriers in cell culture |
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Authors: | Anand Sundararajan Lu-Kwang Ju |
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Institution: | (1) Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325-3906, USA;(2) Present address: Martek Biosciences Corp., 555 Rolling Hills Lane, Winchester, KY 40391, USA |
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Abstract: | The gas vesicles isolated from the cells of filamentous cyanobacterium Anabaena flos-aquae were treated and sterilized with glutaraldehyde and then evaluated for their effectiveness as gas carriers in cell culture.
Anchorage-dependent Vero cells were grown in a packed bed of microcarrier beads under the perfusion of Dulbecco’s Modified
Eagle’s Medium with 1% serum. The culture medium supplemented with 1.8% (v/v) gas vesicles was found to support a 30% higher
maximum glucose utilization rate than the same medium without gas vesicles. The gas vesicle suspension was confirmed to have
no apparent effects on cell metabolism in T-flask cultures. The study results indicated that the gas vesicles, with high oxygen
carrying capacity, can be used to increase the oxygen supply in cell culture systems. |
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Keywords: | Cyanobacteria Flocculation Gas vesicle Microcarrier culture Oxygen supply Vero cells |
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