AXONAL TRANSPORT OF LABELLED PROTEINS IN SENSORY FIBRES OF RABBIT VAGUS NERVE IN VITRO |
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Authors: | W. G. McLean, M. Frizell, J. Sjö strand |
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Affiliation: | Institute of Neurobiology, University of Göteborg, Fack, S-400 33 Göteborg, Sweden |
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Abstract: | —Rabbit vagus nerves and nodose ganglia were incubated in vitro for up to 24 h in two-compartment chambers. After the introduction of [3H]leucine or [3H]fucose to the ganglion compartments a rapid anterograde axonal transport of labelled proteins or glycoproteins occurred at rates of 330 ± 44 mm/day and 336 ± 30 mm/day respectively. Accumulation of [3H]leucine-labelled proteins proximal to a ligature on the nerve was unaffected by a delay of up to 6 h between removal of the nerve and labelling in vitro. Accumulation was prevented by inhibition of protein synthesis in the ganglion but not in the axon and was inhibited in a graded manner by colchicine. |
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