Micro method for determination of borohydride with NAD+ |
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| Authors: | D A Werner C C Huang D Aminoff |
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| Affiliation: | 1. Department of Internal Medicine (Simpson Memorial Institute), The University of Michigan, Ann Arbor, Michigan 48104 USA;2. Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48104 USA |
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| Abstract: | A spectrophotometric method for the determination of borohydride is described. It involves the reduction of NAD+ to a number of isomeric forms of NADH by borohydride. In the standard assay procedure herewith presented, there is a direct proportionality between the absorption at 340 nm and the amount of borohydride in solution over the range 10–100 nmoles, with an effective “molar extinction coefficient” of 12.2 × 103. The method is simple, rapid, and sensitive. |
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