Purification and Partial Characterization of Polysaccharides From Coconut Milk |
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Authors: | WHITE, ALAN R. ELMORE, H. WAYNE WATSON, MARK B. GILL, JAMES P. |
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Affiliation: | * Department of Botany, North Dakota State University Fargo, ND 58105-5517, USA Department of Biological Sciences, Marshall University Huntingdon, WV 25701, USA |
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Abstract: | Ethanol-precipitated polysaccharides of the liquid endospermof coconut, Cocos nucifera L., were composed predominantly ofgalactose and arabinose with minor amounts of mannose and glucose.Gel filtration chromatography on Bio-Gel A-0.5 m revealed asingle major peak (Peak A) at the void volume and a minor peak(Peak B) partially included in the column volume. Peak A containedsome uronosyl residues, but was not susceptible to cleavageby endopolygalacturonase, indicating that it does not containsignificant amounts of polygalacturonic acid. Neutral glycosylresidue composition analysis of Peak A showed that it consistedof 72% galactose and 24% arabinose with minor amounts of glucoseand rhamnose. Coconut milk, Cocos nuciferaL, polysaccharides, glycosyl composition |
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