Somatic embryogenesis and plantlet regeneration from leaf segments of Piper colubrinum |
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Authors: | Yusuf A Tyagi RK Malik SK |
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Institution: | (1) Tissue Culture & Cryopreservation Unit, National Bureau of Plant Genetic Resources, New Delhi, India, 110 012 |
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Abstract: | Direct somatic embryogenesis from in vitro-cultured leaf segments of multiple disease-resistant pepper, Piper colubrinum Link is reported. Somatic embryos were initiated on Murashige and Skoog's (1962) basal medium containing 2.2 μM benzyladenine+0.46
μM kinetin and multiplied profusely through secondary embryogenesis on the same medium. Some 91% somatic embryos converted
into plantlets on MS medium supplemented with 4.4 μM benzyladenine+0.23 μM kinetin and plantlets developed on half-strength
MS+2.4 μM indole-3-butyric acid. Plantlets were hardened, transferred to soil, and 100% of plants survived. Various developmental
stages of somatic embryogenesis were studied using histological methods.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | pepper spice secondary embryogenesis tissue culture |
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