cysB and cysE mutants of Escherichia coli K12 show increased resistance to novobiocin |
| |
Authors: | Jasna Rakonjac Mirjana Milic and Dragutin J Savic |
| |
Institution: | (1) Institute of Molecular Genetics and Genetic Engineering, Vcjvode Stepe 283, PO Box 794, 11001 Belgrade, Yugoslavia;(2) Present address: The Rockefeller University, 10021 New York, NY, USA |
| |
Abstract: | Summary Mutations in the cysB and cysE genes of Escherichia coli K12 cause an increase in resistance to the gyrase inhibitor novobiocin but not to coumermycin, acriflavine and rifampicin. This unusual relationship was also observed among spontaneous novobiocin resistant (Novr) mutants: 10% of Novr mutants isolated on rich (LA) plates with novobiocin could not grow on minimal plates, and among those approximately half were cysB or cysE mutants. Further analyses demonstrated that cysB and cysE negative alleles neither interfere with transport of novobiocin nor affect DNA supercoiling. |
| |
Keywords: | Escherichia coli cysB and cysE genes Resistance to novobiocin |
本文献已被 SpringerLink 等数据库收录! |