Pyruvate kinase isozymic shifts of differentiating chick myogenic cells in vivo and in culture. |
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Authors: | J M Cardenas E Bandman C Walker R C Strohman |
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Institution: | 1. Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA;2. Department of Zoology, University of California, Berkeley, California 94720 USA |
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Abstract: | Developing chick skeletal muscle undergoes an isozymic shift from type K pyruvate kinase to type M during development. A major increase in pyruvate kinase activity follows the isozymic shift, resulting in at least 40-fold higher specific activities by adulthood. Similar isozymic changes occur in primary cultures of myogenic cells from 12-day-old chick embryos. Cultures initially contain only type K pyruvate kinase. Type M appears by the fourth day of culture and accounts for 80–90% of the activity by the eleventh day. Type M did not accumulate when cell fusion was prevented by removing Ca2+ from the growth medium or when protein synthesis was inhibited by cycloheximide. |
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Keywords: | Present address and that to which reprint requests should be sent: Chemistry Department University of North Carolina Chapel Hill North Carolina 27514 |
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