Vertical distribution of bacterial community structure in the sediments of two eutrophic lakes revealed by denaturing gradient gel electrophoresis (DGGE) and multivariate analysis techniques |
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Authors: | Jin Zeng Liuyan Yang Jiayun Li Yi Liang Lin Xiao Lijuan Jiang Dayong Zhao |
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Institution: | (1) State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210093, China |
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Abstract: | Vertical distribution of bacterial community structure was investigated in the sediments of two eutrophic lakes of China,
Lake Taihu and Lake Xuanwu. Profiles of bacterial communities were generated using a molecular fingerprinting technique, denaturing
gradient gel electrophoresis (DGGE) followed by DNA sequence analysis, and the results were interpreted with multivariate
statistical analysis. To assess changes in the genetic diversity of bacterial communities with changing depth, DGGE banding
patterns were analysed by cluster analysis. Distinct clusters were recognized in different sampling stations of Lake Taihu.
Canonical correspondence analysis (CCA) was carried out to infer the relationship between environmental variables and bacterial
community structure. DGGE samples collected at the same sampling site clustered together in both lakes. Total phosphorus,
organic matter and pH were considered to be the key factors driving the changes in bacterial community composition. |
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Keywords: | Bacterial community structure Sediment DGGE Eutrophic lake Multivariate statistical analysis |
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