Fatty-acid composition and biosynthesis in cell suspension cultures of Glycine max (L.) Merr., Catharanthus roseus G. Don and Nicotiana tabacum L. |
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Authors: | J J MacCarthy P K Stumpf |
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Institution: | (1) Department of Biochemistry and Biophysics, University of California, 95616 Davis, CA, USA |
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Abstract: | The fatty-acid composition of C. roseus and N. tabacum cell suspension cultures was unaffected by subculture on Wood and Braun, Murashige and Skoog, or Gamborg B5C media. However, placing the cultures — which were normally grown at 25° C — at 15° C reduced growth but resulted in enhanced formation of oleic and linolenic acids in C. roseus cultures and increased levels of linoleic and linolenic acids in cultures of G. max and N. tabacum, respectively. The incorporation of 14C]acetate into 14C]linoleic acid was more rapid in N. tabacum cells than in G. max cells, but was very poor in C. roseus where the 14C] label was distributed mainly between palmitic and oleic acids. |
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Keywords: | Acetate incorporation Catharanthus Cell suspension cultures Fatty acids Glycihe Nicotiana Temperature and fatty-acid synthesis |
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