Expression in cowpea seedlings of chimeric transgenes after electroporation into seed-derived embryos |
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Authors: | Vani Akella Paul F Lurquin |
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Institution: | (1) Department of Genetics and Cell Biology, Washington State University, 99164-4234 Pullman, WA, USA |
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Abstract: | Summary Cowpea (Vigna unguiculata Walp) embryos mechanically isolated from mature seeds and incubated in the presence of plasmid DNA harboring chimeric gus genes were shown to germinate into seedlings expressing -glucuronidase activity in a variety of tissues, including the apical meristem. Embryo electroporation in the presence of DNA and protectants such as spermine and LipofectinTM increased both the proportion of embryo-derived seedlings expressing the chimeric gene and the level of gene expression. Microscopic observations of thin sections showed that the blue crystals representing the end product of transgene activity on X-glu were exclusively located inside the treated cells. Histological localization of the blue dye crystals varied with the promoter used to drive the transgene. |
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