PCR-CTPP: a rapid and reliable genotyping technique based on ZFX/ZFY alleles for sex identification of tiger (<Emphasis Type="Italic">Panthera tigris</Emphasis>) and four other endangered felids |
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| Authors: | Kun Wei Zhihe Zhang Wenping Zhang Xiao Xu Xu Liang Guangxin He Fujun Shen Liang Zhang Rong Hou Bisong Yue |
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| Institution: | (1) Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan, 610064, P.R. China;(2) Key Laboratory of Endangered Wildlife Breeding and Conservation Genetics of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, 610081, P.R. China;(3) College of Science, Honghe University, Mengzi, Yunnan, 661100, P.R. China |
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| Abstract: | An inexpensive, time-saving and reliable method, polymerase chain reaction with confronting two-pair primers (PCR-CTPP), was
developed for sex identification in tiger (Panthera tigris) based on zinc finger alleles (ZFX/ZFY). A site of “C/G” transversion representing fixed differences that discriminated between
ZFX and ZFY exons among felids was identified for primers designing. This primer set was successfully tested on samples including
blood, shed hairs, dried skin, and stool which contained potential contamination caused by prey DNA. Cross species tests shown
that this primer set was also useful for sex identification in four other endangered felids. |
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| Keywords: | Felids PCR-CTPP Sex identification Tiger ZFX ZFY |
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