New dual electrochemical detector for microbore liquid chromatography determination of dopamine and serotonin in rat striatum dialysates |
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Authors: | Fu-Chou Cheng Ying Shih Yea-Jiuan Liang Lin-Lan Yang Chung-Shi Yang |
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Affiliation: | aDepartment of Medical Research, Taichung Veterans General Hospital, Taichung 402, Taiwan;bDepartment of Chemistry, National Chung-Hsing University, Taichung 402, Taiwan;cDepartment of Applied Cosmetology, Hung-Kuang Institute of Medical and Nursing Technology, Taichung 433, Taiwan |
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Abstract: | A new type of liquid chromatographic (LC) dual thin-layer amperometric detector for the simultaneous measurement of trace levels of dopamine and serotonin in microdialysates is described. The concentrations of these analytes in rat dialysates are usually in the sub-nanomolar concentration range (typically, 0.10–5.00 pg in 5-μl dialysates). With this dual electrode, a glass-lined microbore column provides excellent sensitivity, selectivity, and separation. In addition, a three- to five-fold improvement in anodic current or cathodic responses over conventional dual electrodes in microbore LC can be achieved. Due to the irreversible electrochemical properties of some interference peaks, this dual electrode provides reliable measurement of dopamine based on the cathodic signal. The detection limit (signal-to-noise RATIO=3) of this assay is 0.02 pg per injection for dopamine or serotonin. This new dual electrode allows the simultaneous measurements of basal dopamine and serotonin in rat striatum dialysates without the use of re-uptake inhibitors in perfusion medium. |
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Keywords: | Microdialysis Dopamine Serotonin 5-Hydroxytryptamine |
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