Selective inhibition of free arachidonic acid production in activated alveolar macrophages by calmodulin antagonists |
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Authors: | Y Nakagawa K Waku |
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Affiliation: | Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan. |
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Abstract: | The effect of calmodulin antagonists on the amounts of free fatty acids produced by rabbit alveolar macrophages was determined by fluorometric high-performance liquid chromatography. Opsonized zymosan-induced arachidonic acid production was dramatically suppressed in the presence of W-7 and trifluoperazine without an effect on the production of other fatty acids. Calmodulin antagonists inhibited phospholipase A and abolished the release of arachidonic acid from phospholipids. The present results suggest that a zymosan-sensitive pool of 20:4, which is different from that of other fatty acids, is present in macrophages and that calmodulin antagonists selectively inhibit phospholipase A, which preferentially degrades phospholipids with 20:4. |
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