Genomic DNA sequences of two new genes for new storage protein glutelin in rice. |
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Authors: | F Takaiwa K Oono |
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Institution: | Department of Cell Biology, National Institute of Agrobiological Resources, Ibaraki. |
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Abstract: | A new cDNA and two genomic genes encoding the rice storage protein glutelin were isolated and sequenced. The nucleotide sequence of one gene (GluA-3) was completely identical with that of the new cDNA identified here, and the other (GluA-4) was a pseudogene. These glutelin genes were closely related to each other, and belonged to the subfamily A containing the type I (GluA-1) and II (GluA-2) glutelin genes. The Northern blot analysis, using synthetic oligonucleotide specific to the GluA-3 gene as a probe, showed that this gene was expressed earlier than other glutelin genes during seed maturation. |
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