Callus Induction and Shoot Regeneration from Stem, Rachis and Leaf Explants in Beach Pea (Lathyrus japonicus Willd) |
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| Authors: | S C Debnath D B McKenzie K B McRae |
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| Institution: | 1. Atlantic Food and Horticulture Research Centre, Agriculture and Agri-Food Canada, Kentville, Nova Scotia, B4N 1J5, Canada
2. Atlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada, St. John’s, Newfoundland, A1E 5Y7, Canada
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| Abstract: | Combinations of naphthaleneacetic acid (NAA) and benzyladenine (BA) affected the callus formation from stem, rachis and leaf explants of beach pea. Mature leaf pieces were best for inducing a callus; when cultured on Murashige and Skoog’s (MS) medium supplemented with 4.4μM BA at least 87% of the explants callused regardless of the NAA concentration. Optimal induction was achieved with 5.4 to 10.7μM NAA depending on the explants used. BA at 4.4μM was always more effective than at the lower concentration of 1.1μM. Multiple buds were induced from calli and formed shoots when transferred to MS medium supplemented with 2.7μM NAA + 4.4μM BA. |
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