| 随机扩增多态性对白色念珠菌分型的估价 |
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| 引用本文： | 李冬梅 王瑞礼 李若瑜 朱衡 朱立煌. 随机扩增多态性对白色念珠菌分型的估价[J]. 菌物学报, 1995, 14(2) |
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| 作者姓名： | 李冬梅 王瑞礼 李若瑜 朱衡 朱立煌 |
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| 作者单位： | 北京医科大学真菌和真菌病研究中心 北京 100034；中国科学院遗传所 北京 100101 |
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| 摘 要： | 利用随机扩增多态性(RAPD-PGR)的方法对48株白念珠菌Candida albicans(Robin)Berkh进行了分析。由初步试验中,随机选用了18种引物,筛选出OPA-14引物,该引物扩增的带型清晰可辨,不同菌株之间扩增的带数6—12条不等,共有6条主带。扩增片段的长度粗略估计在300—2000bp左右。除个别菌株的带型相同以外,大多数菌株之间呈多态性分布,其带型的数目和扩增的片段存在差异。对简单的DNA制备方法,以及RAPD-PCR在临床应用和流行病学调查中的可行性进行了初步探讨。
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| 关 键 词： | 白色念珠菌 分型 随机扩增多态性DNA |
ASSESSMENT OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS IN THE CLASSIFICATION OF CANDIDA ALBICANS |
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| Li Dongmei Wang Duanli Li RuoyuResearch Center of Medical Mycology,the First Teaching Hospital,Beijing Medical University Beijing Zhu Heng Zhu LihuangInstitute of Genetics,Academia Sinica,Beijing. ASSESSMENT OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS IN THE CLASSIFICATION OF CANDIDA ALBICANS[J]. Mycosystema, 1995, 14(2) |
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| Authors: | Li Dongmei Wang Duanli Li RuoyuResearch Center of Medical Mycology the First Teaching Hospital Beijing Medical University Beijing Zhu Heng Zhu LihuangInstitute of Genetics Academia Sinica Beijing |
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| Affiliation: | Li Dongmei Wang Duanli Li RuoyuResearch Center of Medical Mycology,the First Teaching Hospital,Beijing Medical University Beijing 100034Zhu Heng Zhu LihuangInstitute of Genetics,Academia Sinica,Beijing 100101 |
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| Abstract: | Randomly amplified polymorphic DNA markers (RAPD-PCR) analysis-was performed in 46 clinic strains and 2 standard strains of Candida albicans. Only the profiles of OPA-14 derived from 18 random primers were very distict. It showed that the amplified numbers of band were from 6 to 12 among different strains. However, there were 6 identical fragments that can be observed in all the amplified profiles of strains, the length of fragments varied from 300 to 2 000bp. Most of strains showed a wide diversity in number and length of amplified fragments. In this paper, the feasibility of application of RAPD-PCR and isolation of fungal DNA by means of benzyl chloride in clinic and epidemiology were also discussed. |
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| Keywords: | Candida albicans Classification Randomly amplified polymorphic DNA markers |
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