Inhibition of human HT-29 colon carcinoma cell adhesion by a 4-fluoro-glucosamine analogue |
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Authors: | Barbara Woynarowska Charles J. Dimitroff Moshewar Sharma Khushi L. Matta Ralph J. Bernacki |
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Affiliation: | (1) Department of Experimental Therapeutics, Roswell Park Cancer Institute, 14263 Buffalo, NY, USA;(2) Department of Gynecologic Oncology, Roswell Park Cancer Institute, 14263 Buffalo, NY, USA;(3) Present address: Department of Radiology, University of Texas Health Science Center at San Antonio, 78284 San Antonio, TX, USA |
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Abstract: | Cell surface glycoconjugates play an important role in cellular recognition and adhesion. Modification of these structures in tumour cells could affect tumour cell growth and behaviour, including metastasis. 2-Acetamido-1,3,6-tri-O-acetyl-4-deoxy-4-fluoro--D-glycopyranose (4-F-GlcNAc) was synthesized as a potential inhibitor and/or modifier of tumour cell glycoconjugates. The effect of this sugar analogue on the adhesive properties of human colon carcinoma HT-29 cells was evaluated. Treatment of HT-29 cells with 4-F-GlcNAc led to reduced cell surface expression of terminal lactosamine, sialyl-Lex and sialyl-Lea, as determined by Western blotting and flow cytometry. The aberrant expression of these oligosaccharide structures on the HT-29 cell surface resulted in: (1) decreased E-selectin mediated adhesion of human colon cells to human umbilical cord endothelial cells (HUVEC); (2) impaired adhesion of HT-29 cells to -galactoside binding lectin, galectin-1; and (3) reduced ability to form homotypic aggregates. After exposure to 4-F-GlcNAc, lysosomal associated membrane proteins (lamp) 1 and 2, and carcinoembryonic antigen (CEA) detected in HT-29 cells were of lower molecular weight, probably due to impaired glycosylation. These results strongly suggest that modification of tumour cell surface molecules can alter tumour cell adhesion and that tumour cell surface oligosaccharides may be suitable targets for therapeutic exploitation.Abbreviations 4-F-GlcNAc 2-acetamido-1,3,6-tri-O-acetyl-4-deoxy-4-fluoro--glucopyranose - GlcNAc N-acetylglucosamine - s-Lex sialyl-Lewisx - s-Lea sialyl-Lewisa - lamp-1 and lamp-2 Lysosomal Associated Membrane Protein 1 and 2 - CEA carcinoembryonic antigen - DMEM Dulbecco's Modified Eagle Medium - PBS Phosphate Buffered Saline (2.7 mM KCl, 1.5 mM KH2PO4, 137 mM NaCl, 6.5 mM Na2HPO4, pH 7.3) - BSA Bovine Serum Albumin - PMSF Phenylmethylsulfonylfluoride - TBS Tris Buffered Saline (10 mM Tris, 20 mM NaCl, pH 7.3) - TCA Trichloroacetic Acid - DSA Datura stramonium agglutinin |
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Keywords: | inhibition of tumour cell adhesion glucosamine analogue carcinoembryonic antigen |
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