Copper-catalyzed DNA damage by ascorbate and hydrogen peroxide: kinetics and yield |
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| Authors: | R Stoewe W A Prütz |
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| Affiliation: | Universit?t Freiburg, Institut für Biophysik und Strahlenbiologie, Federal Republic of Germany. |
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| Abstract: | Time profiles for degradation of DNA via reaction of H2O2 with the DNA-Cu+ complex were analyzed over a wide range of concentrations of the components. The yield of DNA damage per H2O2 molecule is 10 times lower than that obtained with gamma-radiolytically generated .OH radicals. The observations can be explained by a model in which H2O2 reacts, slowly on the one hand with DNA-Cu+ by formation of toxic .OH radicals immediately at the DNA and faster on the other hand with Cu+ in the bulk solution by formation of less toxic Cu(III) intermediates. |
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