|
|||||
|
|
Visualization of interaction between ribosome-inactivating proteins and supercoiled DNA with an atomic force microscope |
|
| Authors: | WU Xiaohua LIU WangyiOUYANG Zhenqian LI Minqian |
| Institution: | 1.Shanghai Institute of Biochemistry; Chinese Academy of Sciences; Shanghai 200031; China 2.Shanghai Institute of Nuclear Research; Shanghai 201800; China |
| Abstract: | The interaction between ribosome-inactivating proteins (RIPs) and supercoiled DNA was observed with an atomic force microscope (AFM). It was found that RIPs can bind to both supercoiled DNA and the unwound double stranded loop region in supercoiled DNA. The RIPs hound to the supercoils can induce the conformational change of supercoiled DNA. Furthermore, the supercoiled DNA was relaxed and cleaved into nick or linear form by RIPs. It indicated that RIP seemed to be a supercoil-dependent DNA binding protein and exhibited the activity of su-percoil-dependent DNA endonuclease. |
| Keywords: | atomic force microscope DNA-protein interaction ribosome-inactivating proteins supercoiled DNA supercoil-dependent endonuclease |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《中国科学：生命科学英文版》浏览原始摘要信息 | |
| 点击此处可从《中国科学：生命科学英文版》下载免费的PDF全文 | |