Isolation and characterisation of the phospholipase B gene of Cryptococcus neoformans var. gattii |
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Authors: | Latouche G Nicolas Sorrell Tania C Meyer Wieland |
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Institution: | Molecular Mycology Laboratory, CIDM, Westmead Hospital, ICPMR, Level 3 Room 3114A, Darcy Road, NSW 2145, Australia. |
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Abstract: | Cryptococcus neoformans var. gattii (serotypes B and C) is a human pathogen, ecologically, biochemically, clinically and genetically different from C. neoformans var. grubii (serotype A) and C. neoformans var. neoformans (serotype D). The phospholipase B (PLB1) gene from serotypes B and C was isolated and characterised. It resembled the serotype A and D genes, with an overall sequence homology of more than 85%. The respective open reading frames were 2236 bp (serotype B) and 2239 bp (serotype C) in length. Each contained six introns and encoded a 68-kDa protein destined for secretion. PLB1 was located on the second smallest chromosome in both serotypes. Gene expression, measured as mRNA, was not regulated by temperature, pH or exogenous nutrients. |
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Keywords: | Phospholipase B gene Serotype B Serotype C mRNA expression Cryptococcus neoformans var gattii |
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