The selective increase or decrease of organellar DNA in generative cells just after pollen mitosis one controls cytoplasmic inheritance |
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Authors: | Noriko Nagata Chieko Saito Atsushi Sakai Haruko Kuroiwa Tsuneyoshi Kuroiwa |
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Institution: | (1) Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan, JP;(2) Kyouritsu Women's Junior College University, Kanda, Tokyo 101-0051, Japan, JP |
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Abstract: | Organellar DNA in mature pollen grains of eight angiosperm species (Actinidia deliciosa Lindl., Antirrhinum majus L., Arabidopsis thaliana (L.) Heynh., Medicago sativa L., Musa acuminata Colla, Pelargonium zonale (L.) L'Hér, Petunia hybrida Vilm. and Rhododendron mucronatum (Blume) G. Don, in which the modes of organellar inheritance have been determined genetically, was observed by fluorescence
microscopy using Technovit 7100 resin sections double-stained with 4′,6-diamidino-2-phenylindole (DAPI) and 3,3′-dihexyloxacarbocyanine
iodide (DiOC6). The eight species were classified into four types, based on the presence or absence of organellar DNA in mature generative
cells: namely (1) type “m+p+”, which has both mitochondrial and plastid DNA (P. zonale), (2) type “m+p–”, which only has mitochondrial DNA (M. acuminata), (3) type “m−p+”, which only has plastid DNA (A. deliciosa, M. sativa, R. mucronatum), and (4) type “m−p−”, which has neither mitochondrial nor plastid DNA (A. majus, A. thaliana, P. hybrida). This classification corresponded to the mode of organellar inheritance determined by genetic analysis. The presence or
absence of mitochondrial and plastid DNA corresponded to paternal/biparental inheritance or maternal inheritance of the respective
organelle, respectively. When organellar DNA was present in mature generative cells (m+ or p+), the DNA content of the organelles
in the generative cells started to increase immediately after pollen mitosis one (PMI). In contrast, the DNA content of organelles
in generative cells decreased rapidly after PMI when organellar DNA was absent from mature generative cells (m− or p−). These
results indicate that the modes of inheritance (paternal/biparental inheritance or maternal inheritance) of mitochondria and
plastids are determined independently of each other in young generative cells just after PMI.
Received: 22 December 1998 / Accepted: 8 February 1999 |
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Keywords: | : Cytoplasmic inheritance 3 3′ -dihexyl-oxacarbocyanine iodide Generative cell Organellar DNA Pollen |
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