| 4个甘蓝型油菜品种一步法快速繁殖体系的建立 |
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| 引用本文： | 洪森荣,尹明华,王艾平,徐卫红,石文娟,王昱.4个甘蓝型油菜品种一步法快速繁殖体系的建立[J].亚热带植物科学,2008,37(3):46-48. |
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| 作者姓名： | 洪森荣 尹明华 王艾平 徐卫红 石文娟 王昱 |
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| 作者单位： | 上饶师范学院 生命科学系, 江西 上饶 334001 |
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| 基金项目： | 江西上饶师范学院院级科技项目 |
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| 摘 要： | 采用单因子试验和植物组织培养方法,对4个甘蓝型油菜品种无菌体系的建立以及带芽茎段的增殖和生根进行初步研究。结果表明,4个品种油菜种子最佳的消毒方式为70%酒精消毒30s,再用0.1%氯化汞消毒8~10min;带芽茎段增殖的最佳培养基为MS+6-BA 2mg/L+NAA 1~2mg/L;最佳生根培养基为MS+NAA 2~5mg/L。
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| 关 键 词： | 甘蓝型油菜 种子 带芽茎段 增殖 生根 |
| 收稿时间： | 2008-01-02 |
Rapid Propagation System of 4 Genotype of Brassica napus |
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| HONG Sen-rong,YIN Ming-hua,WANG Ai-ping,XU Wei-hong,SHI Wen-juan,WANG Yu.Rapid Propagation System of 4 Genotype of Brassica napus[J].Subtropical Plant Science,2008,37(3):46-48. |
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| Authors: | HONG Sen-rong YIN Ming-hua WANG Ai-ping XU Wei-hong SHI Wen-juan WANG Yu |
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| Institution: | Department of Life Science, Shangrao Normal College, Shangrao 334001, Jiangxi China |
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| Abstract: | Single factor test and plant tissue culture methods were applied to establish the aseptic system of Brassica napus and study the proliferation and rooting of stem with a bud. The best disinfection way of Brassica napus seed was using 70% ethyl alcohol for 30 seconds then using 0.1% HgCl2 for 8~10 minutes. MS+6-BA 2mg/L+NAA 1~2mg/L gave the best result for proliferation of Brassica napus stem with a bud and MS+NAA 2~5mg/L was best for rooting. |
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| Keywords: | Brassica napus seed stem with a bud proliferation rooting |
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