A selenium-containing single-chain abzyme with potent antioxidant activity. |
| |
| Authors: | Delin You Xiaojun Ren Yan Xue Guimin Luo Tongshu Yang Jiacong Shen |
| |
| Institution: | Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, P R China. |
| |
| Abstract: | Reactive oxygen species (ROS) are products of normal metabolic activities and are thought to be the cause of many diseases. A selenium-containing single-chain abzyme 2F3 (Se-2F3-scFv) that imitates glutathione peroxidase has been produced which has the capacity to remove ROS. To evaluate the antioxidant ability of Se-2F3-scFv, we constructed a ferrous sulfate/ascorbate (Vc/Fe2+)-induced mitochondrial damage model system and investigated the capacity of Se-2F3-scFv to protect mitochondria from oxidative damage. Se-2F3-scFv markedly decreased mitochondrial swelling, inhibited lipid peroxidation, and maintained the activity of cytochrome c oxidase, in comparison with Ebselen, a well-studied glutathione peroxidase mimic, indicating that Se-2F3-scFv has potential for treating diseases mediated by ROS. |
| |
| Keywords: | |
|
|
