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| 一株高产碱性内切聚半乳糖醛酸酶生产菌的筛选与鉴定 | |
| 引用本文： | 李祖明,张洪勋,薛文通,张保国,李鸿玉,张晓岚.一株高产碱性内切聚半乳糖醛酸酶生产菌的筛选与鉴定[J].生物技术通报,2010(2). |
| 作者姓名： | 李祖明 张洪勋 薛文通 张保国 李鸿玉 张晓岚 |
| 作者单位： | 1. 北京联合大学应用文理学院,北京,100191;中国科学院生态环境研究中心,北京,100085;中国农业大学食品科学与营养工程学院,北京,100083 2. 中国科学院生态环境研究中心,北京,100085 3. 中国农业大学食品科学与营养工程学院,北京,100083 4. 北京联合大学师范学院,北京,100011 5. 玉门油田分公司炼油化工总厂,玉门,735200 |
| 基金项目： | 北京市教委科研计划项目，北京市属市管高校人才强教计划资助项目 |
| 摘 要： | 从碱性土样中经分离筛选得到1株固态发酵产碱性内切聚半乳糖醛酸酶活力较高的菌株。经提酶条件优化得到较优的酶液提取提条件为30倍Na2CO3/NaHCO3缓冲液提取1 h。从形态特征、培养特征、生理生化特征以及分子生物学鉴定结果来看,该菌株属B.gibsonii。 |
| 关 键 词： | 碱性内切聚半乳糖醛酸酶 筛选 固态发酵 提取条件优化 鉴定 |
Screening and Identification of a Bacterium with High Yield of Alkaline Endopolygalacturonase |
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| Li Zuming,Zhang Hongxun,Xue Wentong,Zhang Baoguo,Li Hongyu,Zhang Xiaolan.Screening and Identification of a Bacterium with High Yield of Alkaline Endopolygalacturonase[J].Biotechnology Bulletin,2010(2). | |
| Authors: | Li Zuming Zhang Hongxun Xue Wentong Zhang Baoguo Li Hongyu Zhang Xiaolan |
| Abstract: | A bacterial strain highly producing alkaline endopolygalacturonase by solid-state fermentation was obtained from a sampie of alkaline soil.The recovery ratio of the enzyme from the isolate was the highest after 1 h of extraction in 30 volume of Na_2CO_3/NaHCO_3 buffer at 30℃.The isolate was confirmed to be a strain of B.gibsonii after identified by colony characteristics,morphological characteristics,physiologica and biochemical characteristics and molecular biology methods. |
| Keywords: | Alkaline endopolygalacturonase Screening Solid-state fermentation Optimization of extraction condition Identi fication |
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