Simultaneous determination of serum retinol and α- and γ-tocopherol levels in type II diabetic patients using high-performance liquid chromatography with fluorescence detection |
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Authors: | Andrzej Sobczak Barbara Skop Boguslaw Kula |
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Institution: | Department of Chemistry and Biochemistry, Silesian Medical Academy, Medyków 18, 40-752 Katowice, Poland |
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Abstract: | This paper presents a simple reversed-phase high-performance liquid chromatographic method for the simultaneous determination of retinol, and α- and γ-tocopherols in human serum using a fluorescence detector. For chromatographic separation a binary gradient was used: phase A; acetonitrile–butanol (95:5); phase B; water, at a flow-rate of 1.5 ml/min. Serum retinol, and α- and γ-tocopherol levels were measured in patients with non-insulin-dependent diabetes mellitus. Small sample requirement, good reproducibility and sensitivity make this method useful for the determination of the serum levels of these compounds in patients with diabetes mellitus. |
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Keywords: | Vitamins Retinol Tocopherol |
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