Identification of nitrogen-fixing <Emphasis Type="Italic">Paenibacillus</Emphasis> from different plant rhizospheres and a novel <Emphasis Type="Italic">nifH</Emphasis> gene detected in the <Emphasis Type="Italic">P. stellifer</Emphasis> |
| |
Authors: | H J Jin R Tu F Xu S F Chen |
| |
Institution: | 1.State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing,People’s Republic of China;2.Sanfeng Chen. State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing,China |
| |
Abstract: | A total of 534 isolates were selectively obtained from different plant rhizospheres based on their growth on nitrogen-free
medium and their resistance to 80°C for 15 min. Of the 534 isolates, 23 isolates had nifH gene and exhibited nitrogenase activities. Based on 16S rDNA sequence, G + C content assay and DNA-DNA hybridization, the
23 isolates which divided into four monophyletic clusters were all belonged to the Paenibacillus genus. nifH gene deduced amino acid alignment aLnalysis revealed that cluster I, including 15 isolates, showed the highest NifH identity
with Paenibacillus genus; while cluster II identified as P. stellifer by DNA-DNA hybridization was consistent with four uncultured bacterial clones. This study suggested that the nitrogen-fixing
Paenibacillus were distributed in various ecosystems and prevalent in different plant rhizospheres. It was the first demonstration that
nitrogen fixation existed in P. jamilae and P. stellifer. In eight isolates identified as P. stellifer species, a novel nifH gene was detected in Paenibacillus. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|