The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm |
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Authors: | Hiromitsu Fukuzawa Li-Hua Yu Chikage Umeda-Hara Michito Tagawa Hirofumi Uchimiya |
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Affiliation: | (1) Department of Agricultural Chemistry, Shiraoka Research Station of Biological Science, Nissan Chemical Industries, 1470 Shiraoka, Minamisaitama, Saitama, 349-0294, Japan;(2) Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-0032, Japan |
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Abstract: | We generated transgenic tobacco and rice plants harboring a chimeric gene consisting of the 5-upstream sequence of the rice metallothionein gene (ricMT) fused to the -glucuronidase (GUS) gene. The activity and tissue-specific expression of the ricMT promoter were demonstrated in these transgenic plants. In the transgenic rice plants, despite substantial levels of GUS activity in the shoot and root, almost no GUS signal was detected in the endosperm. Thus, the ricMT promoter could be useful in avoiding accumulation of undesired proteins in the seed endosperm. |
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Keywords: | Endosperm Gene promoter Metallothionein Rice Tobacco |
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