Metabolic flux analysis of carbon balance in Lactobacillus strains |
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| Authors: | Yixing Zhang Fan Zeng Keith Hohn Praveen V Vadlani |
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| Institution: | 1. Bioprocessing and Renewable Energy Laboratory, Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas;2. Dept. of Chemical Engineering, Kansas State University, Manhattan, Kansas;3. Dept. of Grain Science and Industry, Bioprocessing and Renewable Energy Laboratory, Kansas State University, Manhattan, Kansas |
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| Abstract: | Metabolic flux analyses were performed based on the carbon balance of six different Lactobacillus strains used in this study. Results confirmed that L. delbrueckii, L. plantarum ATCC 21028, L. plantarum NCIMB 8826 ΔldhL1, L. plantarum NCIMB 8826 ΔldhL1‐pCU‐PxylAB, and L. plantarum NCIMB 8826 ΔldhL1‐pLEM415‐xylAB metabolized glucose via EMP: whereas, L. brevis metabolized glucose via PK pathway. Xylose was metabolized through the PK pathway in L. brevis, L. plantarum NCIMB 8826 ΔldhL1‐pCU‐PxylAB and L. plantarum NCIMB 8826 ΔldhL1‐pLEM415‐xylAB. Operation of both EMP and PK pathways was found in L. brevis, L. plantarum NCIMB 8826 ΔldhL1‐pCU‐PxylAB, and L. plantarum NCIMB 8826 ΔldhL1‐pLEM415‐xylAB when glucose plus xylose were used as carbon source. The information of detailed carbon flow may help the strain and biomass selection in a designed process of lactic acid biosynthesis. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1397–1403, 2016 |
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| Keywords: | Lactobacillus plantarum Lactobacillus delbrueckii Lactobacillus brevis phosphoketolase pathway Embden‐Meyerhof pathway |
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