Production of an anti‐Candida peptide via fed batch and ion exchange chromatography |
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| Authors: | Rudra Palash Mukherjee Robert Beitle Srinivas Jayanthi T.K.S. Kumar David S. McNabb |
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| Affiliation: | 1. Ralph E. Martin Dept. of Chemical Engineering, University of Arkansas, Fayetteville, AR;2. Dept. of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR;3. Dept. of Biological Sciences, University of Arkansas, Fayetteville, AR |
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| Abstract: | Interest in peptides as diagnostic and therapeutic materials require their manufacture via either a recombinant or synthetic route. This study examined the former, where a recombinant fusion consisting of an antifungal peptide was expressed and isolated from Escherichia coli. Fed batch fermentation with E. coli harboring an arabinose‐inducible plasmid produced the 12 residue anti‐Candida peptide fused to the N‐terminal of Green Fluorescent Protein (GFPUV). The purification of the fusion protein, using ion‐exchange chromatography, was monitored by using the intrinsic fluorescence of GFPUV. The recombinant antifungal peptide was successfully released by cyanogen bromide‐induced cleavage of the fusion protein. The recombinant peptide showed the expected antifungal activity. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:865–871, 2016 |
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| Keywords: | antifungal peptide fed batch cyanogen bromide |
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