Determination of the structure of sulfated tetra- and pentasaccharides obtained by alkaline borohydride degradation of hen ovomucin. A fast atom bombardment-mass spectrometric and1H-NMR spectroscopic study |
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| Authors: | Gérard Strecker Jean-Michel Wieruszeski Claude Martel Jean Montreuil |
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| Affiliation: | (1) Laboratoire de Chimie Biologique (Laboratoire National et Unité Associée au C.N.R.S. no. 217) de l'Université des Sciences et Techniques de Lille Flandres-Artois, F-59655 Villeneuve d'Ascq, France |
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| Abstract: | Alkaline borohydride reductive cleavage of hen ovomucin resulted in the release of a series of neutral and acidic oligosaccharide-alditols.1H-NMR spectroscopy in combination with fast ion bombardment-mass spectrometry in negative ion mode were used for investigation of the structures of three oligosaccharide-alditols. The following structures were established:Abbreviations NeuAc N-acetyl-d-neuraminic acid - Gal d-galactose - GlcNAc N-acetyl-d-glucosamine - Gal-NAc-ol N-acetyl-d-galactosaminitol - NMR nuclear magnetic resonance - FAB-MS fast atom bombardmentmass spectrometry |
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| Keywords: | ovomucin sulfated oligosaccharides fast atom bombardment-mass spectrometry 1H-NMR |
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