Detection of Human Polyomavirus Dna In Papanicolaou Stained Smears of Urinary Sediment By In Situ Hybridization |
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Authors: | N. IWA T. K. KOBAYASHI C. YUTANI |
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Affiliation: | Department of Pathology, National Cardiovascular Center, Osaka, Japan. |
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Abstract: | Papanicolaou stained smears of urinary sediment containing inclusion bearing urothelial cells suggestive of human polyomavirus infection were destained and reprocessed for in situ hybridization using a biotinylated probe for human polyomavirus DNA. Seven slides were processed in this way. A hybridization signal for viral DNA was noted in each case, even in smears that had previously been stored for 11 years. This simple and rapid non-radioactive detection system is a valuable supplement to routine urinary cytology for the definitive diagnosis of this virus infection. |
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Keywords: | human polyomavirus inclusion bearing transitional cells in situ hybridization destained Papanicolaou smears |
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