Direct shoot regeneration from leaf segments of mature plants of <Emphasis Type="Italic">Echinacea purpurea</Emphasis> (L.) moench |
| |
| Authors: | Subbaiah?M?Mechanda Email author" target="_blank">Bernard?R?BaumEmail author Douglas?A?Johnson John?T?Arnason |
| |
| Institution: | (1) Eastern Cereal and Oilseed Research Centre, Agriculture & AgriFood Canada, Central Experimental Farm, KIA 0C6 Ottawa, ON, Canada;(2) Ottawa-Carleton Institute of Biology, University of Ottawa, 30 Marie Curie, K1A OC7 Ottawa, ON, Canada |
| |
| Abstract: | Summary This is the first communication of direct shoot regeneration from fully developed leaves of potted mature Echinacea purpurea plants. Shoot buds were induced directly on the adaxial surface of mature leaf tissues of E. purpurea 30 d after culture initiation on Woody Plant Medium (WPM) supplemented with various levels of 6-benzyladenine (BA). Maximum
shoot organogenesis, with 12–20 shoots per leaf segment, was obtained with 5% coconut milk and 2.5 mg l−1 (6μM) BA in 30 d. Callus was induced using 0.5 mgl−1 (1μM) α-naphthaleneacetic acid and 2.5 mgl−1 (6μM) BA. The regenerated shoots were rooted on WPM supplemented with 1.5 mgl−1 (3μM) of indole-3-butyric acid, 3% sucrose, and 0.85% agarose. Rooted plants were successfully transferred to soil in pots and
appeared morphologically normal and flowered in a growth chamber. |
| |
| Keywords: | callus Echinacea medicinal plant micropropagation regeneration root shoot |
| 本文献已被 SpringerLink 等数据库收录！ |
|
