Callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (Triticum aestivum L.) |
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| Authors: | Rita Harris Martha Wright Michael Byrne James Varnum Blanche Brightwell Karel Schubert |
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| Institution: | (1) Monsanto Agricultural Products Co., 700 Chesterfield Village Parkway, 63198 Chesterfield Village, MO, USA;(2) Present address: EniChem Americas, 2000 Princeton Park Corporate Center, 08852 Monmouth Junction, NJ, USA;(3) Present address: CibaGeigy, Ltd., P.O. Box 12257, 27709-2257 Research Triangle Park, NC, USA;(4) Present address: Genetics Institute, 87 Cambridge Park Drive, 02140 Cambridge, MA, USA;(5) Present address: Chemistry Department, Cornell University, 14853 Ithaca, NY, USA;(6) Present address: Department of Biology, Washington University, 63130 St. Louis, MO, USA |
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| Abstract: | Protoplasts were isolated from anther-derived suspension cultures of commercial wheat (Triticum aestivum L. cv. Chris). The protoplasts were released enzymatically and isolated by centrifugation on a sucrose cushion. The isolated protoplasts were initially cultured in a liquid medium in the dark. Numerous microcalli were produced under these conditions, some of which differentiated into globular embryos. Upon transfer to a solid medium and exposure to 16h/8h light/dark cycle, the protocalli proliferated and many of the somatic embryos matured. Complete plantlets were obtained and maintained in sterile culture.Abbreviations 2,4-D
2,4-Dichlorophenoxyacetic acid
- MES
2-N-morpholino] ethanesulfonic acid |
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