Colorimetric assay of liver and Escherichia coli asparatate transcarbamylases in the presence of 2-mercaptoethanol |
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| Authors: | C R Savage J M Schumer H Weinfeld |
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| Affiliation: | Department of Medicine C, Roswell Park Memorial Institute and the Department of Biochemistry, Roswell Park Graduate Division, State University of New York at Buffalo, Buffalo, New York 14203 USA |
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| Abstract: | A modification of the method of Prescott and Jones (1) for the colorimetric determination of carbamyl aspartate has been developed to permit the assay of aspartate transcarbamylases in the presence of 2-mercaptoethanol. Interference by this compound is eliminated by means of N-ethylmaleimide. The usefulness of the modified method is illustrated by examination of the contrasting properties of the Escherichia coli and rat liver enzymes. |
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| Keywords: | To whom requests for reprints should be addressed. |
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