Lamivudine production via enantioselective deamination by thermostable Bacillus caldolyticus cytidine deaminase |
| |
Authors: | Ju-Hyung Woo Hyun-Jeung Shin Tae-Ho Kim Sa-Youl Ghim Lak-Shin Jeong Jong-Guk Kim Bang-Ho Song |
| |
Affiliation: | (1) Departments of Microbiology, Korea;(2) Biology Education, Kyungpook National University, 702-701 Taegu, Korea;(3) College of Pharmacy, Ewha Womans University, 120-750 Seoul, Korea |
| |
Abstract: | To decrease the costs of producing the anti-HIV drug, lamivudine, an enzymatic conversion process was developed instead of the traditional chemical method. Thermostable cytidine deaminase was over-produced by cloning the cdd gene into E. coli JF611/pCJH53 from Bacillus caldolyticus. The purified cytidine deaminase was recovered from the lysate of the recombinant E. coli JF611/pCJH53 by removing heat-denatured proteins and eluting sequential chromatography. When the enzyme was used to deaminate (–)--l-(2R, 5S)- and (+)--d-(2S, 5R)-1, 3-oxathiolanyl-cytosine, about 68% of the (+)--d-(2S, 5R)-1, 3-oxathiolanyl-cytosine was deaminated into the corresponding (+)-thiauridine maximally. |
| |
Keywords: | Bacillus caldolyticus cdd cytidine deaminase lamivudine 3TC |
本文献已被 SpringerLink 等数据库收录! |
|