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Complete nucleotide sequence of pTZ12, a chloramphenicol-resistance plasmid of Bacillus subtilis
Authors:T Aoki  N Noguchi  M Sasatsu  M Kono
Institution:1. USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, Crop Bioprotection Research Unit, 1815 N. University St., Peoria, IL 61614, USA;2. USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, Mycotoxin Prevention and Applied Microbiology Research Unit, 1815 N. University St., Peoria, IL 61614, USA;1. Department of Molecular Biology and Genetics, Section for Crop Genetics and Biotechnology, Aarhus University, Forsogsvej 1, 4200 Slagelse, Denmark;2. Wageningen University, 6708PB Wageningen, The Netherlands
Abstract:The complete nucleotide sequence of pTZ12, a chloramphenicol-resistance (CmR) plasmid (2517 bp) derived from Corynebacterium xerosis plasmid pTZ10, has been determined after propagation in Bacillus subtilis. The nucleotide sequence of pTZ12 suggests that a recombination event may have occurred naturally within the open reading frames for the Rep protein of pT181 (or a pT181-like plasmid) and pC221 (or a pC221-like plasmid).
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