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Application of cellulase and hemicellulase to pure xylan, pure cellulose, and switchgrass solids from leading pretreatments |
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| Authors: | Shi Jian Ebrik Mirvat A Yang Bin Garlock Rebecca J Balan Venkatesh Dale Bruce E Pallapolu V Ramesh Lee Y Y Kim Youngmi Mosier Nathan S Ladisch Michael R Holtzapple Mark T Falls Matthew Sierra-Ramirez Rocio Donohoe Bryon S Vinzant Todd B Elander Richard T Hames Bonnie Thomas Steve Warner Ryan E Wyman Charles E |
| Affiliation: | a Center for Environmental Research and Technology, Bourns College of Engineering, University of California at Riverside, 1084 Columbia Avenue, Riverside, CA 92507, USA b Biomass Conversion Research Laboratory, Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USA c Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI, USA d Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, USA e LORRE, Department of Agricultural and Biological Engineering, Purdue University, 500 Central Dr., West Lafayette, IN 47907, USA f Department of Chemical Engineering, Texas A&M University, 3122 TAMU, College Station, TX 77843-3122, USA g Universidad de los Andes Chemical Engineering Department Grupo de Conversion de Energia, Bogotá, Colombia h Chemical and Biosciences Center, National Renewable Energy Laboratory, 1617 Cole Blvd., Golden, CO 80401, USA i Ceres, Inc., 1535 Rancho Conejo Blvd, Thousand Oaks, CA 91320, USA j Genencor, a Danisco Division, 925 Page Mill Road, Palo Alto, CA 94304, USA |
| Abstract: | Accellerase 1000 cellulase, Spezyme CP cellulase, β-glucosidase, Multifect xylanase, and beta-xylosidase were evaluated for hydrolysis of pure cellulose, pure xylan, and switchgrass solids from leading pretreatments of dilute sulfuric acid, sulfur dioxide, liquid hot water, lime, soaking in aqueous ammonia, and ammonia fiber expansion. Distinctive sugar release patterns were observed from Avicel, phosphoric acid swollen cellulose (PASC), xylan, and pretreated switchgrass solids, with accumulation of significant amounts of xylooligomers during xylan hydrolysis. The strong inhibition of cellulose hydrolysis by xylooligomers could be partially attributed to the negative impact of xylooligomers on cellulase adsorption. The digestibility of pretreated switchgrass varied with pretreatment but could not be consistently correlated to xylan, lignin, or acetyl removal. Initial hydrolysis rates did correlate well with cellulase adsorption capacities for all pretreatments except lime, but more investigation is needed to relate this behavior to physical and compositional properties of pretreated switchgrass. |
| Keywords: | Pretreatment Enzymatic hydrolysis Biofuels Adsorption Xylooligomers |
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