Saccharomyces cerevisiae ACR2 gene encodes an arsenate reductase |
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Authors: | Rita Mukhopadhyaya Barry P Rosena |
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Affiliation: | Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201, USA |
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Abstract: | The ACR2 gene of Saccharomyces cerevisiae was disrupted by insertion of a HIS3 gene. Cells with the disruption were sensitive to arsenate. This phenotype could be complemented by ACR2 on a plasmid. The ACR2 gene was cloned and expressed in Escherichia coli as a malE gene fusion with a C-terminal histidine tag. The combination of chimeric MBP-Acr2-6H protein and yeast cytosol from an ACR2-disrupted strain exhibited arsenate reductase activity. |
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Keywords: | Yeast Arsenate resistance |
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