Synergistic effects of focus ultrasound with different frequency and hematoporphyrin on human tumor cells |
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Authors: | QI HaoTAN ShengjiangMA YuyingSONG Cunniu |
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Institution: | (1) College of Life Science, Shaanxj Normal University, 710062 Xi’an, China;(2) Applied Acoustic Institute, Shaanxi Normal University, 710062 Xi’an, China |
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Abstract: | Human hematopoietic cell K562, human melenonla cell LiBr and human stomach cancer cells were exposed to ultrasound (US, 1.75 W/cm2, 1.4, 2.16 and 2.4 MHz)in vitro in the presence or absence of hematoporphyrin (Hp, 100 μg/mL). The cell damaging effects of treatments were determined by
means of the Trypan Blue dye exclusion test, MTT test and FDA test. The experimental results showed that the same cell line
had different sensibilities to the US of different frequencies, and different cell line had different damage at the same acoustical
radiation. The cornbined treatment with US and Hp enhanced greatly the cell damage, and no sensibility of insonation cells
to US with Hp was observed. The cell damage tests showed that the results of MTT test corresponded well with that of Trypan
Blue dye test.
Project supported by the Natural Science Foundation of Shaanxi Province |
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Keywords: | ultrasound hematoporphyrin human tumor cell antitumor effect cell culture |
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