微生物转化浮萍资源生产蛋白酶的条件

利用微生物转化富含蛋白质的浮萍生产蛋白酶,为浮萍的高值化利用开辟一条新途径。在摇瓶转化的基础上,探索在5L发酵罐上沙雷氏菌(Serratiasp.)SYBC H转化浮萍生产蛋白酶的最佳转化条件。通过对温度、通风量、搅拌转速、pH等参数的研究,发现在5L发酵罐上,沙雷氏菌SYBC H转化浮萍生产蛋白酶的最佳条件为通风量6vvm,搅拌转速400r/min,发酵温度30℃。转化过程中,采用全自动控制pH值(9.0),蛋白酶的产量比不控制pH的对照组提高了23.3%。进一步研究发现,利用微生物沙雷氏菌SYBC H转化浮萍生产的蛋白酶具有耐有机溶剂的特性,在某些亲水性有机溶剂中保留90%以上的活性,是一种稀少珍贵的极端酶。

Conditions for microbiological transformation of duckweed resources into protease

Novel microbial transformation methods are explored for high-value utilization of protein-rich duckweed to produce proteases.Based on flask transformation,the transformation conditions for the production of proteases from duckweed by Serratia sp.SYBC H were optimized in a 5 L fermentor.Through study on temperature,ventilation volume,stirring speed and pH,it is found that the best transformation conditions were ventilation volume 6 vvm,stirring speed 400 r/min,culture temperature 30 ℃.The protease production with constant auto-feed control of pH 9.0 during process was 23.3％ higher than that from pH non-control process.Further studies have shown that the protease produced from duckweed by Serratia sp.SYBC H still retained more than 90％ activity in some hydrophilic orga-nic solvents,making it a rare and precious extreme protease.