电针对海洛因成瘾大鼠PAG的NPY表达的影响

目的:观察韩氏穴位神经刺激器(HNAS)针刺对海洛因成瘾大鼠中脑导水管周围灰质(PAG)的神经肽Y(NPY)表达的影响。方法:按给药剂量逐日递增的原则皮下注射海洛因建立成瘾模型,Morris水迷宫测大鼠空间学习记忆,免疫组化测大鼠PAG的NPY表达。结果:①成瘾组大鼠在定位航行实验中逃避潜伏期及搜索距离较正常组明显延长(P0.05),而针刺组较成瘾组明显缩短(P0.05);空间探索实验中,成瘾组原平台所在象限搜索时间及原平台所在象限的游泳距离占总距离的百分比较正常组明显缩短(P0.05),针刺组较成瘾组明显增加(P0.01)。②PAG的NPY表达成瘾组低于正常组(P0.05),针刺组高于成瘾组(P0.05)。结论:HANS针刺对海洛因成瘾引起的大鼠学习记忆减退有恢复作用,并上调其PAG的NPY表达。

Effect of HANS electroacupuncture on the expression of NPY in PAG of heroin addicted rats

Objective：To examine the effects of Han＇s acupoint and nerve stimulator（HANS） electroacupuncture on the expression of NPY in periaqueductal grey（PAG） of heroin addicted rats. Methods： Heroin was injected subcutaneously according to the principle of daily increasing dose in rats of experimented group. The ability of special learning and memory were tested by Morris water maze; The expression of NPY in PAG of rat were detected by immunohistochemistry. Results： ①Escape latency and searching distance in heroin-addiction group were significantly increased compared with those of normal group during the place navigation test （P0.05）. However, in acupuncture group, escape latency and searching distance was obviously shortened compared with those of heroin-addiction group（P0.05）. The exploring time and distance of original platform area in proportion to the total distance in heroin-addiction group significantly decreased compared with those of normal group during spatial probe test（P0.05）. The exploring time and distance of original platform area in proportion to the total distance in acupuncture group was increased compared with those in heroin-addiction group（P0.01）. ②The expression of NPY of heroin-addiction group was lower than that in normal group in PAG, while those of acupuncture group was higher than that in the heroin-addiction group （P0.05）. Conclusion： The learning and memory induced by heroin-addiction could be reversed and the expression of NPY in PAG was increased by HANS in rats.