JUVENILE HORMONE BINDING PROTEIN IN THE OVARIES OF HOUSEFLY MUSCA DOMESTICA VACINA |
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Authors: | Wenhui Zheng Qianjun Li He Gong |
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Institution: | Institute of Zoology, Acadrmia Sinica, Bkijing 100080, China |
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Abstract: | Abstract By using charcocal binding assay, the juvenile hormone binding protein (JHBP) was determined in the ovaries of houseflies. This ovarian JHBP possesses high affinity with juvenile hormone III (JH III) and has a Kd of 2.1 III 10--8 M. The binding of 3H-juvenile hormone III (3H-JH III) to this protein was inhibited by unlablled JH III, but not by juvenile hormone analog ZR 512 or ZR 515. The level of this ovarian JHBP reached the highest in houseflies 48 h after emergence, and was 6. 5-fold and 15. 5-fold higher than that in housefIies 60 h and 72 h after emergence, respectively. No binding activity was detected in the ovaries of houseflies 24 h or 36 h after emergence. The absence of JHBP in the ovaries of houseflies 36 h after emergence could be reversed by applying JH III to newly emerged houseflies. The data suggest that the fluctuation of the JHBP concentration might associate with the action of juvenile hormone (JH) on housefly vitellogenesis. |
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Keywords: | juvenile hormone (JH) juvenile hormone binding protein (JHBP) ovary Musca domesti ca vacina |
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