Effect of heparin on the stimulation of non-vascular cells by human acidic and basic FGF |
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| Authors: | S Uhlrich O Lagente M Lenfant Y Courtois |
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| Institution: | 1. C.N.R.S, Institut de Chimie des Substances Naturelles, 91190 Gif-sur-Yvette, France;2. INSERM - U118, C.N.R.S. LA 630, 29 Rue Wilhem, 75016 Paris, France;1. Department of Central Laboratory, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, 272000, China;2. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China;3. Ministry of Education Center for Food Safety of Animal Origin, Dalian University of Technology, Dalian, 116620, China;1. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China;2. National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China;3. Hypothalamic-Pituitary Research Center, Xiangya Hospital, Central South University, Changsha, Hunan, China;4. Bio-Intelligent Manufacturing and Living Matter Bioprinting Center, Research Institute of Tsinghua University in Shenzhen, Tsinghua University, Shenzhen, China;5. Reproductive Medicine Center, Xiangya Hospital, Central South University, Hunan, China |
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| Abstract: | We have purified acidic and basic fibroblast growth factors from human brain (h-aFGF, h-bFGF) and studied the effect of heparin on the growth stimulation by these factors of hamster fibroblast CC139 cells and bovine epithelial lens (BEL) cells. In both the presence and the absence of foetal calf serum (FCS) heparin cooperates with h-aFGF in a dose dependent manner to stimulate both types of cells. The cooperation with h-bFGF is much less. An unpurified human brain fraction containing both factors behaves differently: in the absence of FCS, heparin enhances the activity of the crude fraction on BEL cells, while in the presence of FCS, it decreases this activity. These results indicate that heparin cooperates strongly with h-aFGF to stimulate non-vascular cell proliferation while in a partially purified extract and in the presence of serum it can induce the opposite effect. |
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