Isolation and characterization of pigment mutants from a filamentous cyanobacterium |
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Authors: | A K Mishra D N Tiwari |
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Institution: | (1) Centre of Advanced Study in Botany, Banaras Hindu University, 221 005 Varanasi, India |
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Abstract: | Five strains of a pigment mutant were isolated following UV irradiation and N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) mutagenesis
from a non-nitrogen fixing mutant of the cyanobacteriumGloeotrichia ghosei. Two of them (B-1 and V-1) were isolated by UV mutagenesis and other three (B-3, B-7 and Br-6) by MNNG mutagenesis. Among
the five strains cultures of three strains (B-1, B-3 and B-7) were typically blue-green in colour. Culture of strain V-1 was
found to be violet-pink and of Br-6 was brownish in colour. The parent strain of these mutants was dark-blue in colour. Blue-green
mutants showed the predominance of phycocyanin (610 nm) whereas violet-pink and brown strains showed the predominance of phycoerythrin
(550 nm) in the absorption spectra of water-soluble pigments. In contrast to these strains their parent strain showed both
the absorption peaks (at 550 and 610 nm). Occurrence of stable pigment mutants of a filamentous cyanobacterium indicates that
the synthesis of water-soluble pigments is genetically controlled in these mutant strains. |
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